Phytosterols can be found free (FS) or conjugated as steryl esters (SEs), steryl glucosides (SGs), or acylated steryl glucosides (ASGs). The content of conjugated sterols may vary greatly between species, tissues, and growing conditions. SG are primarily located in the plasma membrane, but their function is not yet fully known; they seem to play an important role in the regulation of the biophysical properties of the plasma membrane. The synthesis of SG is catalysed by UDP-glucose: sterol-glucosyltransferases (SGT). Studies suggest that plants contain multigenic families that codify SGT isoenzymes with specialised functions. In this study a system for the expression of isozyme SlSGT1 in Eschericha coli has been developed, so that the recombinant enzyme can be subsequently purified, and its enzymatic activity characterised.