In the production of meat chickens it is necessary to complement their diet with selenium in order to ensure optimum growth, reproduction, and health. In the intestine, the main function of selenium is the synthesis of different selenoproteins, such as glutathione peroxidase (GSH-Px) and selenoprotein P1 (SEPP1), which are involved in redox balance. The overall objective of this work was to study the effects of a new organic selenium source compared to an inorganic source in a Caco-2 intestinal cell model. The complementation of cultures with the organic source resulted in an increase in GSH-Px activity, SEPP1 protein expression, and GSH-Px2 genetic expression, as well as a protective effect against hydrogen peroxide. Therefore, it can be concluded that this organic source modifies the activity and expression of different selenoproteins that exert a protector role against oxidative stress, and thus it could be considered a good dietary supplement for the poultry.