Educació en competències: TFG-Farmàcia
Aubets, Eva; Ciudad, Carlos J.
Abstract
Replication stress activates the ATR-CHK1 pathway to produce cell cycle arrest and provide enough time to repair DNA damage. Alteration of this checkpoint can lead to the development of illnesses such as cancer. In this study, we investigated the inhibition of genes involved in the ATR-CHK1 pathway, like WEE1 and CHK1, using polypurine reverse Hoogsteen hairpins (PPRHs).
Five PPRHs directed against different regions of WEE1 and CHK1 showed a high decrease in cell viability in different cell lines. Furthermore, inhibition of both genes triggered an increase in the percentage of apoptotic cells.
We concluded that the inhibition of components from the ATR-CHK1 pathway by PPRHs was proven to be highly effective, and therefore may constitute a possible new approach in anti-cancer gene therapy.
Keywords: PPRH, càncer, silenciament gènic, apoptosi.
Reception date: 18/10/2017
Acceptance date: 27/10/2017